What 5‑Amino‑1MQ Is & How It Works
5‑Amino‑1MQ (5‑Amino‑1‑methylquinolinium) is a small‑molecule research compound that targets a key metabolic enzyme called nicotinamide N‑methyltransferase (NNMT). NNMT consumes nicotinamide, a building block in the NAD⁺ salvage pathway — which is essential for cellular energy, mitochondrial function, and metabolic balance. By selectively inhibiting NNMT, 5‑Amino‑1MQ helps preserve nicotinamide for NAD⁺ synthesis, boosting NAD⁺‑dependent cellular pathways like mitochondrial biogenesis, energy metabolism, and sirtuin signaling that are foundational to metabolic regulation.

Mechanism of Action & Metabolic Impact
NNMT inhibition by 5‑Amino‑1MQ has been shown in preclinical adipocyte and animal models to increase intracellular NAD⁺ levels, which supports oxidative phosphorylation and energy expenditure rather than storage. This shift enhances fat oxidation, improves glucose handling and insulin sensitivity, and helps maintain lean muscle metabolism under metabolic stress. Specific research data indicate that NNMT inhibition significantly reduces intracellular 1‑methylnicotinamide (1‑MNA) levels, elevates NAD⁺ by ~1.2–1.6× in cell models, and suppresses lipogenesis pathways linked to adiposity.

Research Findings & Investigational Benefits
In diet‑induced obese rodent studies, 5‑Amino‑1MQ has been associated with reduced adipose tissue mass, enhanced energy expenditure, and improved insulin sensitivity — all without changes in food intake. These preclinical outcomes also extend to improved mitochondrial function and metabolic flexibility, which are core endpoints in metabolic health research. While robust human clinical trials are still lacking, these mechanisms form the basis for ongoing metabolic and longevity‑focused investigations into fat regulation, NAD⁺‑associated signaling, and cellular energy dynamics.